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KMID : 0900920010250010063
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Korean journal of Animal Reproduction
2001 Volume.25 No. 1 p.63 ~ p.69
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Effect of nitric Oxide Compounds on the Development of Porcine IVM/IVF Embryos
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Park K.-E.
Park Choon-Keun
Kim Choung-Ik
Cheong Hee-Tae
Park Dong-Heon
Yang Boo-Keun
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Abstract
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This study was carried out to examine the effects of nitric oxide compounds (hemoglobin and L-NAME) on the development of porcine in vitro maturation (IVM) and in vitro fertilization (IVF) oocytes. Cumulus cell free embryos derived from porcine IVM/IVF oocytes were cultured in NCSU23 medium containing 1~5 mutextrm{g}/mell hemoglobin added to 44 and 96hrs in culture times, and in NCSU23 medium containing 0, 10, 50 or 100mM L-NAME. The developmental rates beyond morulae stage in 0, 1 and 5 mutextrm{g}/mell hemoglobin groups add to 44hrs in vitro culture times were 52.4%, 57.6% and 57.4%, respectively. The addition of hemoglobin groups made it slightly higher than the control group. The proportion of embryos developed to morulae and blastocysts in 1 mutextrm{g}/mell hemoglobin add to 96hrs after in vitro culture (70.8%) was a little higher than those of 0 and 5 mutextrm{g}/mell hemoglobin (66.2% and 62.8%). There was no significant difference in all groups (P〉0.05). The developmental rates beyond morulae stage in 0, 10, 50 and 100mM of L-NAME groups add to 96hrs after in vitro culture were 65.2%, 73.5%, 70.1% and 53.3%, respectively 10mM and 50mM L-NAME groups were significantly higher than in 0 and 100mM of L-NAME groups (P＜0.05). In conclusions, these results indicate that L-NAME (10mM, 50mM) can increase the proportion of embryos that develop into morulae and blastocysts but hemoglobin did not affect.
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KEYWORD
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Nitric oxide, Serum, Porcine, IVM/IVF embryo, Cleavage rates
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